Rv2241 Pyruvate dehydrogenase E1 component (EC 1.2.4.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2241 aceE Pyruvate dehydrogenase E1 component (EC 1.2.4.1) CDS 2512539 2515244 + 2 706 901 FALSE

Rv2241 (Pyruvate dehydrogenase E1 component (EC 1.2.4.1)) is predicted to be co-regulated in modules bicluster_0324 with residual 0.52 and bicluster_0458 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.01 for bicluster_0324 and 0.15 and 30,000.00 for bicluster_0458 respectively.

These modules are enriched for following go terms: organonitrogen compound metabolic proces..., primary metabolic process, small molecule metabolic process, small molecule biosynthetic process, single-organism biosynthetic process, organonitrogen compound biosynthetic pro....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-1.539 54 2512398 2512452 2512539
Last update: 10/16/2017 - 12:06
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18441 MT2301 2854
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0324
e.value: 
0.0045
Motif Bicluster: 
e.value: 
0.014
Motif Bicluster: 
0.52
bicluster_0458
e.value: 
0.15
Motif Bicluster: 
e.value: 
30000
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Pyruvate dehydrogenase E1 component pyruvate dehydrogenase subunit E1
Operon # Operon
1471
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Citrate cycle (TCA cycle)

35
Total items in this category:  

KEGG

Valine, leucine and isoleucine biosynthesis

21
Total items in this category:  

KEGG

Pyruvate metabolism

40
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609378 NP_216757.1 Run
GO:0004739

pyruvate dehydrogenase (acetyl-transferring) activity

pyruvate dehydrogenase (acetyl-transferring) activity

Details: 
Catalysis of the reaction: pyruvate + lipoamide = S-acetyldihydrolipoamide + CO2.
GO Category: 
molecular_function
6
Total items in this category:  
GO:0000287

magnesium ion binding

magnesium ion binding

Details: 
Interacting selectively and non-covalently with magnesium (Mg) ions.
GO Category: 
molecular_function
52
Total items in this category:  
GO:0004739

pyruvate dehydrogenase (acetyl-transferring) activity

pyruvate dehydrogenase (acetyl-transferring) activity

Details: 
Catalysis of the reaction: pyruvate + lipoamide = S-acetyldihydrolipoamide + CO2.
GO Category: 
molecular_function
6
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0045254

pyruvate dehydrogenase complex

pyruvate dehydrogenase complex

Details: 
Complex that carries out the oxidative decarboxylation of pyruvate to form acetyl-CoA; comprises subunits possessing three catalytic activities: pyruvate dehydrogenase (E1), dihydrolipoamide S-acetyltransferase (E2), and dihydrolipoamide dehydrogenase (E3).
GO Category: 
cellular_component
4
Total items in this category:  
GO:0055114

oxidation-reduction process

oxidation-reduction process

Details: 
A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO Category: 
biological_process
19
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: