Rv2259 Formaldehyde dehydrogenase MscR, NAD/mycothiol-dependent (EC 1.2.1.66) / S-nitrosomycothiol reductase MscR

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2259 adhE2 Formaldehyde dehydrogenase MscR, NAD/mycothiol-dependent (EC 1.2.1.66) / S-nitrosomycothiol... CDS 2532245 2533330 + 1 086 361 FALSE

Rv2259 (Formaldehyde dehydrogenase MscR, NAD/mycothiol-dependent (EC 1.2.1.66) / S-nitrosomycothiol reductase MscR) is predicted to be co-regulated in modules bicluster_0496 with residual 0.57 and bicluster_0585 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0496 and 0.06 and 350.00 for bicluster_0585 respectively.

These modules are enriched for following go terms: sulfur compound metabolic process cofactor biosynthetic process, coenzyme metabolic process, cofactor metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0496
e.value: 
2.9e-38
Motif Bicluster: 
e.value: 
6.4e-27
Motif Bicluster: 
0.57
bicluster_0585
e.value: 
0.055
Motif Bicluster: 
e.value: 
350
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Probable zinc-dependent alcohol dehydrogenase AdhE2 zinc-dependent alcohol dehydrogenase AdhE2
Operon # Operon
1484 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609396 NP_216775.1 Run
GO:0010127

mycothiol-dependent detoxification

mycothiol-dependent detoxification

Details: 
The chemical reactions using mycothiol to convert an alkylating agent to an S-conjugate of mycothiol. The latter is cleaved to release mercapturic acid which is excreted from the cell.
GO Category: 
biological_process
2
Total items in this category:  
GO:0016491

oxidoreductase activity

oxidoreductase activity

Details: 
Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.
GO Category: 
molecular_function
12
Total items in this category:  
GO:0050607

mycothiol-dependent formaldehyde dehydrogenase activity

mycothiol-dependent formaldehyde dehydrogenase activity

Details: 
Catalysis of the reaction: formaldehyde + mycothiol + NAD+ = S-formylmycothiol + NADH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0055114

oxidation-reduction process

oxidation-reduction process

Details: 
A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO Category: 
biological_process
19
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.440000 0.95

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: