Rv2281 Probable low-affinity inorganic phosphate transporter

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2281 pitB Probable low-affinity inorganic phosphate transporter CDS 2553173 2554831 + 1 659 552 FALSE

Rv2281 (Probable low-affinity inorganic phosphate transporter) is predicted to be co-regulated in modules bicluster_0156 with residual 0.52 and bicluster_0319 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 180.00 and 890.00 for bicluster_0156 and 0.16 and 8.30 for bicluster_0319 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Putative phosphate permease Rv2281_MT2339 phosphate ABC transporter permease
Operon # Operon
1499
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609418 NP_216797.1 Run
GO:0006817

phosphate ion transport

phosphate ion transport

Details: 
The directed movement of phosphate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
GO Category: 
biological_process
4
Total items in this category:  
GO:0005315

inorganic phosphate transmembrane transporter activity

inorganic phosphate transmembrane transporter activity

Details: 
Catalysis of the transfer of a inorganic phosphate from one side of a membrane to the other, up its concentration gradient. The transporter binds the solute and undergoes a series of conformational changes. Transport works equally well in either direction and is driven by a chemiosmotic source of energy. Chemiosmotic sources of energy include uniport, symport or antiport.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0016020

membrane

membrane

Details: 
Double layer of lipid molecules that encloses all cells, and, in eukaryotes, many organelles; may be a single or double lipid bilayer; also includes associated proteins.
GO Category: 
cellular_component
33
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.030000 1.24

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: