Rv2285 Diacyglycerol O-acyltransferase (EC 2.3.1.20)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2285 Diacyglycerol O-acyltransferase (EC 2.3.1.20) CDS 2557473 2558810 + 1 338 445 FALSE

Rv2285 (Diacyglycerol O-acyltransferase (EC 2.3.1.20)) is predicted to be co-regulated in modules bicluster_0091 with residual 0.52 and bicluster_0326 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 12.00 for bicluster_0091 and 0.00 and 0.40 for bicluster_0326 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18788 MT2343 3031
Product (LegacyBRC) Product (RefSeq)
UPF0089 protein Rv2285_MT2343
Operon # Operon
1501 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Diacylglycerol O-acyltransferase Glycerolipid metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609422 NP_216801.1 Run
GO:0004144

diacylglycerol O-acyltransferase activity

diacylglycerol O-acyltransferase activity

Details: 
Catalysis of the reaction: acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
GO Category: 
molecular_function
6
Total items in this category:  
GO:0045017

glycerolipid biosynthetic process

glycerolipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glycerolipids, any lipid with a glycerol backbone.
GO Category: 
biological_process
7
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.390000 1.58

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: