Rv2297

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2297 CDS 2568438 2568890 + 453 150 FALSE

Rv2297 () is predicted to be co-regulated in modules bicluster_0281 with residual 0.48 and bicluster_0579 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 140.00 and 1,200.00 for bicluster_0281 and 940.00 and 3,500.00 for bicluster_0579 respectively.

These modules are enriched for following go terms: acetate-CoA ligase activity tetrapyrrole metabolic process, tetrapyrrole biosynthetic process, organonitrogen compound biosynthetic pro..., heterocycle biosynthetic process, IMP biosynthetic process, 'de novo' IMP biosynthetic process, IMP metabolic process, organic cyclic compound biosynthetic pro....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv2297_MT2354
Operon # Operon
1509 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics
Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609434 NP_216813.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.620000 3.72

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: