Rv2301 Cutinase Cut2 (EC 3.1.1.74)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2301 cut2 Cutinase Cut2 (EC 3.1.1.74) CDS 2573015 2573707 + 693 230 FALSE

Rv2301 (Cutinase Cut2 (EC 3.1.1.74)) is predicted to be co-regulated in modules bicluster_0371 with residual 0.54 and bicluster_0556 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.10 and 3.90 for bicluster_0371 and 0.02 and 1.40 for bicluster_0556 respectively.

These modules are enriched for following go terms: anion transport cobalamin binding.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15737 MT2358 2106
Product (LegacyBRC) Product (RefSeq)
Probable cutinase cut2 cutinase CUT2
Operon # Operon
1513
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116961 NP_216817.2 Run
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0042783

active evasion of host immune response

active evasion of host immune response

Details: 
Any mechanism of immune avoidance that directly affects the host immune system, e.g. blocking any stage in host MHC class I and II presentation. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
20
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.780000 2.50

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: