Rv2325c Transmembrane component of energizing module of ECF transporters in Mycobacteria

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2325c Transmembrane component of energizing module of ECF transporters in Mycobacteria CDS 2597009 2597857 - 849 282 FALSE

Rv2325c (Transmembrane component of energizing module of ECF transporters in Mycobacteria) is predicted to be co-regulated in modules bicluster_0359 with residual 0.50 and bicluster_0405 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0359 and 0.58 and 2.90 for bicluster_0405 respectively.

These modules are enriched for following go terms: alcohol metabolic process, phospholipid metabolic process, phospholipid biosynthetic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv2325c_MT2387
Operon # Operon
1531 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

ABC transporters

57
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609462 NP_216841.1 Run
GO:0006824

cobalt ion transport

cobalt ion transport

Details: 
The directed movement of cobalt (Co) ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
GO Category: 
biological_process
1
Total items in this category:  
GO:0015087

cobalt ion transmembrane transporter activity

cobalt ion transmembrane transporter activity

Details: 
Catalysis of the transfer of cobalt (Co) ions from one side of a membrane to the other.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0009236

cobalamin biosynthetic process

cobalamin biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of cobalamin (vitamin B12), a water-soluble vitamin characterized by possession of a corrin nucleus containing a cobalt atom.
GO Category: 
biological_process
5
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.460000 0.79

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: