Rv2334 Cysteine synthase (EC 2.5.1.47)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2334 cysK1 Cysteine synthase (EC 2.5.1.47) CDS 2608796 2609728 + 933 310 FALSE

Rv2334 (Cysteine synthase (EC 2.5.1.47)) is predicted to be co-regulated in modules bicluster_0162 with residual 0.54 and bicluster_0251 with residual 0.46.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 45.00 and 5,400.00 for bicluster_0162 and 780.00 and 7,200.00 for bicluster_0251 respectively.

These modules are enriched for following go terms: cysteine biosynthetic process from serin..., cysteine biosynthetic process, cysteine metabolic process, L-serine metabolic process cysteine metabolic process, L-serine metabolic process, serine family amino acid biosynthetic pr..., sulfur amino acid biosynthetic process, small molecule biosynthetic process, single-organism biosynthetic process, sulfur amino acid metabolic process, alpha-amino acid biosynthetic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Rv2334 cysteine synthase A CysK1
Operon # Operon
1539 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Cysteine synthase Sulfur metabolism, Cysteine and methionine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Cysteine and methionine metabolism

28
Total items in this category:  

KEGG

Selenocompound metabolism

40
Total items in this category:  

KEGG

Sulfur metabolism

12
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116972 YP_177868.1 Run
GO:0004124

cysteine synthase activity

cysteine synthase activity

Details: 
Catalysis of the reaction: O3-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0004124

cysteine synthase activity

cysteine synthase activity

Details: 
Catalysis of the reaction: O3-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0030170

pyridoxal phosphate binding

pyridoxal phosphate binding

Details: 
Interacting selectively and non-covalently with pyridoxal 5' phosphate, 3-hydroxy-5-(hydroxymethyl)-2-methyl4-pyridine carboxaldehyde 5' phosphate, the biologically active form of vitamin B6.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.490000 0.98

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: