Rv2343c DNA primase (EC 2.7.7.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2343c dnaG DNA primase (EC 2.7.7.-) CDS 2620533 2622452 - 1 920 639 FALSE

Rv2343c (DNA primase (EC 2.7.7.-)) is predicted to be co-regulated in modules bicluster_0117 with residual 0.46 and bicluster_0242 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 6,200.00 and 9,100.00 for bicluster_0117 and 0.00 and 0.00 for bicluster_0242 respectively.

These modules are enriched for following go terms: DNA modification.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
DNA primase DNA primase
Operon # Operon
1547 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

DNA replication

14
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609480 NP_216859.1 Run
GO:0006260

DNA replication

DNA replication

Details: 
The cellular metabolic process in which a cell duplicates one or more molecules of DNA. DNA replication begins when specific sequences, known as origins of replication, are recognized and bound by initiation proteins, and ends when the original DNA molecule has been completely duplicated and the copies topologically separated. The unit of replication usually corresponds to the genome of the cell, an organelle, or a virus. The template for replication can either be an existing DNA molecule or RNA.
GO Category: 
biological_process
2
Total items in this category:  
GO:0008270

zinc ion binding

zinc ion binding

Details: 
Interacting selectively and non-covalently with zinc (Zn) ions.
GO Category: 
molecular_function
15
Total items in this category:  
GO:0003676

nucleic acid binding

nucleic acid binding

Details: 
Interacting selectively and non-covalently with any nucleic acid.
GO Category: 
molecular_function
13
Total items in this category:  
GO:0003677

DNA binding

DNA binding

Details: 
Any molecular function by which a gene product interacts selectively with DNA (deoxyribonucleic acid).
GO Category: 
molecular_function
37
Total items in this category:  
GO:0006304

DNA modification

DNA modification

Details: 
The covalent alteration of one or more nucleotide sites in DNA, resulting in a change in its properties.
GO Category: 
biological_process
5
Total items in this category:  
GO:0003896

DNA primase activity

DNA primase activity

Details: 
Catalysis of the synthesis of a short RNA primer on a DNA template, providing a free 3'-OH that can be extended by DNA-directed DNA polymerases. Catalyzed by a DNA-directed RNA polymerase that forms a complex with alpha DNA polymerase.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: