Rv2349c Phospholipase C 3 PlcC (EC 3.1.4.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2349c plcC Phospholipase C 3 PlcC (EC 3.1.4.3) CDS 2627172 2628698 - 1 527 508 FALSE

Rv2349c (Phospholipase C 3 PlcC (EC 3.1.4.3)) is predicted to be co-regulated in modules bicluster_0368 with residual 0.52 and bicluster_0424 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 430.00 and 1,000.00 for bicluster_0368 and 0.00 and 0.00 for bicluster_0424 respectively.

These modules are enriched for following go terms: cellular component biogenesis, cellular component organization or bioge..., protein binding cellular macromolecule catabolic process, nucleobase-containing compound catabolic..., aromatic compound catabolic process, macromolecule catabolic process, exonuclease activity, active with either..., deoxyribonuclease activity, oxidoreductase activity, acting on paire..., exonuclease activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-13464 MT2414 6
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, ArsR family
No 36 -0.34 0.200395 CDS
Transcriptional regulator, PadR family
No -134 0.1 0.97031 CDS
Possible transcriptional regulatory protein
No -126 0.47 0.0995724 CDS
Motif 1 Motif 2 Residual
bicluster_0368
e.value: 
430
Motif Bicluster: 
e.value: 
1000
Motif Bicluster: 
0.52
bicluster_0424
e.value: 
1.4e-35
Motif Bicluster: 
e.value: 
7.6e-26
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Phospholipase C 3 phospholipase C 3 PLCC
Operon # Operon
1552
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Inositol phosphate metabolism

9
Total items in this category:  

KEGG

Glycerophospholipid metabolism

21
Total items in this category:  

KEGG

Ether lipid metabolism

7
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609486 NP_216865.1 Run
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0034480

phosphatidylcholine phospholipase C activity

phosphatidylcholine phospholipase C activity

Details: 
Catalysis of the reaction: phosphatidylcholine + H2O = 1,2-diacylglycerol + a choline phosphate.
GO Category: 
molecular_function
4
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.090000 0.96

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: