Rv2350c Phospholipase C 3 PlcC (EC 3.1.4.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2350c plcB Phospholipase C 3 PlcC (EC 3.1.4.3) CDS 2628781 2630319 - 1 539 512 FALSE

Rv2350c (Phospholipase C 3 PlcC (EC 3.1.4.3)) is predicted to be co-regulated in modules bicluster_0015 with residual 0.46 and bicluster_0368 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2,500.00 and 2,900.00 for bicluster_0015 and 430.00 and 1,000.00 for bicluster_0368 respectively.

These modules are enriched for following go terms: DNA modification cellular component biogenesis, cellular component organization or bioge..., protein binding.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.585 30 2630376 2630346 2630319
Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18703 MT2415 2861
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0015
e.value: 
2500
Motif Bicluster: 
e.value: 
2900
Motif Bicluster: 
0.46
bicluster_0368
e.value: 
430
Motif Bicluster: 
e.value: 
1000
Motif Bicluster: 
0.52
Product (LegacyBRC) Product (RefSeq)
Phospholipase C 2 membrane-associated phospholipase C
Operon # Operon
1553
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Inositol phosphate metabolism

9
Total items in this category:  

KEGG

Glycerophospholipid metabolism

21
Total items in this category:  

KEGG

Ether lipid metabolism

7
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609487 NP_216866.1 Run
GO:0004629

phospholipase C activity

phospholipase C activity

Details: 
Catalysis of the reaction: a phospholipid + H2O = 1,2-diacylglycerol + a phosphatidate.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0034480

phosphatidylcholine phospholipase C activity

phosphatidylcholine phospholipase C activity

Details: 
Catalysis of the reaction: phosphatidylcholine + H2O = 1,2-diacylglycerol + a choline phosphate.
GO Category: 
molecular_function
4
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.820000 2.38

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: