Rv2356c PPE family protein

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2356c PPE40 PPE family protein CDS 2637688 2639535 - 1 848 615 FALSE

Rv2356c (PPE family protein) is predicted to be co-regulated in modules bicluster_0029 with residual 0.45 and bicluster_0071 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.30 and 4.20 for bicluster_0029 and 0.04 and 0.76 for bicluster_0071 respectively.

These modules are enriched for following go terms: nucleobase-containing compound catabolic..., aromatic compound catabolic process, macromolecule catabolic process, cellular nitrogen compound catabolic pro..., heterocycle catabolic process, organic cyclic compound catabolic proces..., deoxyribonuclease activity, exonuclease activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18661 MT2425 2791
Product (LegacyBRC) Product (RefSeq)
PPE FAMILY PROTEIN PPE family protein
Operon # Operon
1558
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116976 YP_177872.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.660000 1.37

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: