Rv2357c Glycyl-tRNA synthetase (EC 6.1.1.14)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2357c glyS Glycyl-tRNA synthetase (EC 6.1.1.14) CDS 2639673 2641064 - 1 392 463 FALSE

Rv2357c (Glycyl-tRNA synthetase (EC 6.1.1.14)) is predicted to be co-regulated in modules bicluster_0082 with residual 0.54 and bicluster_0439 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,300.00 and 10,000.00 for bicluster_0082 and 0.00 and 0.00 for bicluster_0439 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.365 66 2641118 2641052 2641064
Product (LegacyBRC) Product (RefSeq)
Glycyl-tRNA synthetase glycyl-tRNA synthetase
Operon # Operon
1559
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Glycine--tRNA ligase Aminoacyl-tRNA biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Aminoacyl-tRNA biosynthesis

69
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609494 NP_216873.1 Run
GO:0004820

glycine-tRNA ligase activity

glycine-tRNA ligase activity

Details: 
Catalysis of the reaction: ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: