Rv2368c Phosphate starvation-inducible protein PhoH, predicted ATPase

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2368c phoH1 Phosphate starvation-inducible protein PhoH, predicted ATPase CDS 2648916 2649974 - 1 059 352 FALSE

Rv2368c (Phosphate starvation-inducible protein PhoH, predicted ATPase) is predicted to be co-regulated in modules bicluster_0314 with residual 0.57 and bicluster_0405 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0314 and 0.58 and 2.90 for bicluster_0405 respectively.

These modules are enriched for following go terms: alcohol metabolic process, phospholipid metabolic process, phospholipid biosynthetic process.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18892 MT2437 3265
Product (LegacyBRC) Product (RefSeq)
Rv2368c phosphate starvation-inducible protein PsiH
Operon # Operon
1564 - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116978 YP_177874.1 Run
GO:0003676

nucleic acid binding

nucleic acid binding

Details: 
Interacting selectively and non-covalently with any nucleic acid.
GO Category: 
molecular_function
13
Total items in this category:  
GO:0016036

cellular response to phosphate starvation

cellular response to phosphate starvation

Details: 
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of phosphate.
GO Category: 
biological_process
2
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.050000 1.33

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: