Rv2384 Bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase MbtA

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2384 mbtA Bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase MbtA CDS 2675936 2677633 + 1 698 565 FALSE

Rv2384 (Bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase MbtA) is predicted to be co-regulated in modules bicluster_0502 with residual 0.52 and bicluster_0527 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 1.20 for bicluster_0502 and 0.47 and 120.00 for bicluster_0527 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
BIFUNCTIONAL ENZYME MBTA: SALICYL-AMP LIGASE [SAL-AMP LIGASE] + SALICYL-S-ArCP SYNTHETASE bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase
Operon # Operon
1572
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Biosynthesis of siderophore group nonribosomal peptides

9
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609521 NP_216900.1 Run
GO:0008668

(2,3-dihydroxybenzoyl)adenylate synthase activity

(2,3-dihydroxybenzoyl)adenylate synthase activity

Details: 
Catalysis of the reaction: 2,3-dihydroxybenzoate + ATP = 2,3-dihydroxybenzoyl 5'-adenylate + diphosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0008668

(2,3-dihydroxybenzoyl)adenylate synthase activity

(2,3-dihydroxybenzoyl)adenylate synthase activity

Details: 
Catalysis of the reaction: 2,3-dihydroxybenzoate + ATP = 2,3-dihydroxybenzoyl 5'-adenylate + diphosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0010106

cellular response to iron ion starvation

cellular response to iron ion starvation

Details: 
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of iron ions.
GO Category: 
biological_process
14
Total items in this category:  
GO:0016881

acid-amino acid ligase activity

acid-amino acid ligase activity

Details: 
Catalysis of the ligation of an acid to an amino acid via a carbon-nitrogen bond, with the concomitant hydrolysis of the diphosphate bond in ATP or a similar triphosphate.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0019290

siderophore biosynthetic process

siderophore biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of siderophores, low molecular weight Fe(III)-chelating substances made by aerobic or facultatively anaerobic bacteria, especially when growing under iron deficient conditions. The complexes of Fe(3+)-siderophores have very high stability constants and are taken up by specific transport systems by microorganisms; the subsequent release of iron requires enzymatic action.
GO Category: 
biological_process
6
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.870000 1.45

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: