Rv2385 Acetyl hydrolase MbtJ

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2385 mbtJ Acetyl hydrolase MbtJ CDS 2677729 2678649 + 921 306 FALSE

Rv2385 (Acetyl hydrolase MbtJ) is predicted to be co-regulated in modules bicluster_0497 with residual 0.53 and bicluster_0527 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 310.00 and 800.00 for bicluster_0497 and 0.47 and 120.00 for bicluster_0527 respectively.

These modules are enriched for following go terms: 3-hydroxyacyl-CoA dehydrogenase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17912 MT2453 543
Product (LegacyBRC) Product (RefSeq)
PUTATIVE ACETYL HYDROLASE MBTJ putative acetyl hydrolase MBTJ
Operon # Operon
1573
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Bisphenol degradation

35
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116980 YP_177876.1 Run
GO:0004806

triglyceride lipase activity

triglyceride lipase activity

Details: 
Catalysis of the reaction: triacylglycerol + H2O = diacylglycerol + a fatty acid anion.
GO Category: 
molecular_function
6
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.740000 1.36

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: