Rv2397c Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2397c cysA1 Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25) CDS 2693909 2694964 - 1 056 351 FALSE

Rv2397c (Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25)) is predicted to be co-regulated in modules bicluster_0462 with residual 0.49 and bicluster_0518 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2,900.00 and 12,000.00 for bicluster_0462 and 410.00 and 4,100.00 for bicluster_0518 respectively.

These modules are enriched for following go terms: peptidoglycan metabolic process, aminoglycan metabolic process, glycosaminoglycan metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Rv2397c sulfate-transport ATP-binding protein ABC transporter CysA1
Operon # Operon
1581 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

ABC transporters

57
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116983 YP_177879.1 Run
GO:0032146

thiosulfate transmembrane-transporting ATPase activity

thiosulfate transmembrane-transporting ATPase activity

Details: 
Catalysis of the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + thiosulfate(out) = ADP + phosphate + thiosulfate(in).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0015419

sulfate transmembrane-transporting ATPase activity

sulfate transmembrane-transporting ATPase activity

Details: 
Catalysis of the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + sulfate(out) = ADP + phosphate + sulfate(in).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0008272

sulfate transport

sulfate transport

Details: 
The directed movement of sulfate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
GO Category: 
biological_process
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: