Rv2404c Translation elongation factor LepA

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2404c lepA Translation elongation factor LepA CDS 2701287 2703248 - 1 962 653 FALSE

Rv2404c (Translation elongation factor LepA) is predicted to be co-regulated in modules bicluster_0271 with residual 0.56 and bicluster_0490 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 0.03 for bicluster_0271 and 0.01 and 730.00 for bicluster_0490 respectively.

These modules are enriched for following go terms: carboxylic acid biosynthetic process, organic acid biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17841 MT2476 292
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -128 0.28 0.33072 Antisense.TSS
No -140 0.28 0.33072 CDS
No -58 0.28 0.33072 Internal.TSS
Motif 1 Motif 2 Residual
bicluster_0271
e.value: 
0.013
Motif Bicluster: 
e.value: 
0.027
Motif Bicluster: 
0.56
bicluster_0490
e.value: 
0.0085
Motif Bicluster: 
e.value: 
730
Motif Bicluster: 
0.57
Product (LegacyBRC) Product (RefSeq)
GTP-binding protein lepA GTP-binding protein LepA
Operon # Operon
1585 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609541 NP_216920.1 Run
GO:0006412

translation

translation

Details: 
The cellular metabolic process in which a protein is formed, using the sequence of a mature mRNA molecule to specify the sequence of amino acids in a polypeptide chain. Translation is mediated by the ribosome, and begins with the formation of a ternary complex between aminoacylated initiator methionine tRNA, GTP, and initiation factor 2, which subsequently associates with the small subunit of the ribosome and an mRNA. Translation ends with the release of a polypeptide chain from the ribosome.
GO Category: 
biological_process
62
Total items in this category:  
GO:0005525

GTP binding

GTP binding

Details: 
Interacting selectively and non-covalently with GTP, guanosine triphosphate.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.60

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: