Rv2416c Enhanced intracellular survival protein

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2416c eis Enhanced intracellular survival protein CDS 2714124 2715332 - 1 209 402 FALSE

Rv2416c (Enhanced intracellular survival protein) is predicted to be co-regulated in modules bicluster_0238 with residual 0.51 and bicluster_0540 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,400.00 and 8,600.00 for bicluster_0238 and 2,700.00 and 15,000.00 for bicluster_0540 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.335 8 2715334 2715326 2715332
Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17913 MT2489 544
Product (LegacyBRC) Product (RefSeq)
Enhanced intracellular survival protein
Operon # Operon
1593
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116987 NP_216932.2 Run
GO:0030649

aminoglycoside antibiotic catabolic process

aminoglycoside antibiotic catabolic process

Details: 
The chemical reactions and pathways resulting in the breakdown of an aminoglycoside antibiotic, any member of a group of broad spectrum antibiotics, of similar toxicity and pharmacology, that contain an aminodeoxysugar, an amino- or guanidino-substituted inositol ring, and one or more residues of other sugars. The group includes streptomycin, neomycin, framycetin, kanamycin, paromomycin, and gentamicin.
GO Category: 
biological_process
1
Total items in this category:  
GO:0034069

aminoglycoside N-acetyltransferase activity

aminoglycoside N-acetyltransferase activity

Details: 
Catalysis of the reaction: acetyl-CoA + aminoglycoside = CoA + N-acetylaminoglycoside.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0044119

growth of symbiont in host cell

growth of symbiont in host cell

Details: 
The increase in size or mass of symbiont, occurring in its host's cell. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
50
Total items in this category:  
GO:0044121

growth of symbiont in host organelle

growth of symbiont in host organelle

Details: 
The increase in size or mass of a symbiont, occurring in its host's organelle. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
5
Total items in this category:  
GO:0052167

modulation by symbiont of host innate immune response

modulation by symbiont of host innate immune response

Details: 
Any process in which an organism modulates the frequency, rate or extent of the innate immune response of the host organism; the innate immune response is the host's first line of defense against infection. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
4
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.40

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: