Rv2436 Ribokinase (EC 2.7.1.15)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2436 rbsK Ribokinase (EC 2.7.1.15) CDS 2733230 2734144 + 915 304 FALSE

Rv2436 (Ribokinase (EC 2.7.1.15)) is predicted to be co-regulated in modules bicluster_0029 with residual 0.45 and bicluster_0140 with residual 0.62.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.30 and 4.20 for bicluster_0029 and 0.00 and 0.00 for bicluster_0140 respectively.

These modules are enriched for following go terms: nucleobase-containing compound catabolic..., aromatic compound catabolic process, macromolecule catabolic process, cellular nitrogen compound catabolic pro..., heterocycle catabolic process, organic cyclic compound catabolic proces..., deoxyribonuclease activity, exonuclease activity .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.328 2733293 2733230
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Lactate-responsive regulator LldR in Enterobacteria, GntR family
No -122 0.42 0.151223 CDS
Beta-carotene ketolase (EC 1.14.-.-)
No -26 -0.09 0.999473 CDS
Motif 1 Motif 2 Residual
bicluster_0029
e.value: 
3.3
Motif Bicluster: 
e.value: 
4.2
Motif Bicluster: 
0.45
bicluster_0140
e.value: 
0.0000061
Motif Bicluster: 
e.value: 
0.000019
Motif Bicluster: 
0.62
Product (LegacyBRC) Product (RefSeq)
RIBOKINASE RBSK ribokinase RBSK
Operon # Operon
1605 Rv2436
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Ribokinase Pentose phosphate pathway
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pentose phosphate pathway

19
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609573 NP_216952.1 Run
GO:0004747

ribokinase activity

ribokinase activity

Details: 
Catalysis of the reaction: ATP + D-ribose = ADP + D-ribose 5-phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.840000 2.48

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.