Rv2438c NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2438c nadE NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase CDS 2734792 2736831 - 2 040 679 FALSE

Rv2438c (NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase) is predicted to be co-regulated in modules bicluster_0009 with residual 0.64 and bicluster_0080 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 6,300.00 and 21,000.00 for bicluster_0009 and 0.00 and 0.00 for bicluster_0080 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

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Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Two component DNA binding Transcriptional regulatory protein TcrA
Repressed -1 -1.32 1.88e-23 Primary.TSS
No -124 -0.38 0.0285438 Primary.TSS
Organic hydroperoxide resistance transcriptional regulator
No -126 0.03 0.935842 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0009
e.value: 
6300
Motif Bicluster: 
e.value: 
21000
Motif Bicluster: 
0.64
bicluster_0080
e.value: 
0.000027
Motif Bicluster: 
e.value: 
0.00045
Motif Bicluster: 
0.51
Product (LegacyBRC) Product (RefSeq)
Rv2438c NAD synthetase
Operon # Operon
1608
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

NAD(+) synthase Nitrogen metabolism, Nicotinate and nicotinamide metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nicotinate and nicotinamide metabolism

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116990 NP_216954.2 Run
GO:0003952

NAD+ synthase (glutamine-hydrolyzing) activity

NAD+ synthase (glutamine-hydrolyzing) activity

Details: 
Catalysis of the reaction: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: