Rv2447c Dihydrofolate synthase (EC 6.3.2.12) / Folylpolyglutamate synthase (EC 6.3.2.17)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2447c folC Dihydrofolate synthase (EC 6.3.2.12) / Folylpolyglutamate synthase (EC 6.3.2.17) CDS 2746135 2747598 - 1 464 487 FALSE

Rv2447c (Dihydrofolate synthase (EC 6.3.2.12) / Folylpolyglutamate synthase (EC 6.3.2.17)) is predicted to be co-regulated in modules bicluster_0504 with residual 0.56 and bicluster_0587 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 3.10 for bicluster_0504 and 0.00 and 0.32 for bicluster_0587 respectively.

These modules are enriched for following go terms: nitrogen cycle metabolic process, transition metal ion binding, hydrolase activity, acting on carbon-nit..., metal ion binding .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE FOLYLPOLYGLUTAMATE SYNTHASE PROTEIN FOLC [FOLYLPOLY-GAMMA-GLUTAMATE SYNTHETASE] [FPGS] folylpolyglutamate synthase protein FolC
Operon # Operon
1614 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Folate biosynthesis

10
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609584 NP_216963.1 Run
GO:0000287

magnesium ion binding

magnesium ion binding

Details: 
Interacting selectively and non-covalently with magnesium (Mg) ions.
GO Category: 
molecular_function
52
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0043531

ADP binding

ADP binding

Details: 
Interacting selectively and non-covalently with ADP, adenosine 5'-diphosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0050897

cobalt ion binding

cobalt ion binding

Details: 
Interacting selectively and non-covalently with a cobalt (Co) ion.
GO Category: 
molecular_function
5
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: