Rv2447c Dihydrofolate synthase (EC 188.8.131.52) / Folylpolyglutamate synthase (EC 184.108.40.206)
Rv2447c (Dihydrofolate synthase (EC 220.127.116.11) / Folylpolyglutamate synthase (EC 18.104.22.168)) is predicted to be co-regulated in modules bicluster_0504 with residual 0.56 and bicluster_0587 with residual 0.58.
These modules are enriched for following go terms: nitrogen cycle metabolic process, transition metal ion binding, hydrolase activity, acting on carbon-nit..., metal ion binding .
This gene is found to be for growth on cholesterol.
|Product (LegacyBRC)||Product (RefSeq)|
|PROBABLE FOLYLPOLYGLUTAMATE SYNTHASE PROTEIN FOLC [FOLYLPOLY-GAMMA-GLUTAMATE SYNTHETASE] [FPGS]||folylpolyglutamate synthase protein FolC|
|BioCyc Gene Page||Cellular Overview Map|
magnesium ion binding
|t-test p-value||Cholesterol/Glycerol Ratio|
How essentiality calculations were done?
The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.
reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.