Rv2467 Membrane alanine aminopeptidase N (EC 3.4.11.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2467 pepN Membrane alanine aminopeptidase N (EC 3.4.11.2) CDS 2768986 2771571 + 2 586 861 FALSE

Rv2467 (Membrane alanine aminopeptidase N (EC 3.4.11.2)) is predicted to be co-regulated in modules bicluster_0272 with residual 0.51 and bicluster_0516 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.38 and 1,700.00 for bicluster_0272 and 2,900.00 and 10,000.00 for bicluster_0516 respectively.

These modules are enriched for following go terms: anion transport, external encapsulating structure, cell periphery, transmembrane transporter activity, transporter activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15003 MT2542 525
Product (LegacyBRC) Product (RefSeq)
PROBABLE AMINOPEPTIDASE N PEPN [LYSYL AMINOPEPTIDASE] [LYS-AP] [ALANINE AMINOPEPTIDASE] aminopeptidase N
Operon # Operon
1627
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Membrane alanyl aminopeptidase Glutathione metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glutathione metabolism

14
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116994 YP_177885.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.870000 2.07

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: