Rv2471 Alpha-glucosidase (EC 3.2.1.20)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2471 aglA Alpha-glucosidase (EC 3.2.1.20) CDS 2773564 2775204 + 1 641 546 FALSE

Rv2471 (Alpha-glucosidase (EC 3.2.1.20)) is predicted to be co-regulated in modules bicluster_0017 with residual 0.60 and bicluster_0587 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.25 and 10.00 for bicluster_0017 and 0.00 and 0.32 for bicluster_0587 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.101 2773642 2773564
Product (LegacyBRC) Product (RefSeq)
PROBABLE ALPHA-GLUCOSIDASE AGLA [MALTASE] [GLUCOINVERTASE] [GLUCOSIDOSUCRASE] [MALTASE-GLUCOAMYLASE] [LYSOSOMAL ALPHA-GLUCOSIDASE] [ACID MALTASE] alpha-glucosidase AglA
Operon # Operon
1630 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Alpha-glucosidase Galactose metabolism, Starch and sucrose metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Galactose metabolism

14
Total items in this category:  

KEGG

Starch and sucrose metabolism

20
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609608 NP_216987.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.850000 1.47

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: