Rv2471 Alpha-glucosidase (EC 3.2.1.20)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2471 aglA Alpha-glucosidase (EC 3.2.1.20) CDS 2773564 2775204 + 1 641 546 FALSE

Rv2471 (Alpha-glucosidase (EC 3.2.1.20)) is predicted to be co-regulated in modules bicluster_0017 with residual 0.60 and bicluster_0587 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.25 and 10.00 for bicluster_0017 and 0.00 and 0.32 for bicluster_0587 respectively.

These modules are enriched for following go terms: .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.101 2773642 2773564
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
No 0 -0.04 0.883634 Antisense.TSS
Mycofactocin system transcriptional regulator
No -144 0.09 0.999588 Antisense.TSS
Motif 1 Motif 2 Residual
bicluster_0017
e.value: 
0.25
Motif Bicluster: 
e.value: 
10
Motif Bicluster: 
0.60
bicluster_0587
e.value: 
0.000002
Motif Bicluster: 
e.value: 
0.32
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
PROBABLE ALPHA-GLUCOSIDASE AGLA [MALTASE] [GLUCOINVERTASE] [GLUCOSIDOSUCRASE] [MALTASE-GLUCOAMYLASE] [LYSOSOMAL ALPHA-GLUCOSIDASE] [ACID MALTASE] alpha-glucosidase AglA
Operon # Operon
1630 Rv2470 - Rv2471
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Alpha-glucosidase Galactose metabolism, Starch and sucrose metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Galactose metabolism

14
Total items in this category:  

KEGG

Starch and sucrose metabolism

20
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609608 NP_216987.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.850000 1.47

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.