Rv2497c Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2497c pdhA Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) CDS 2810993 2812096 - 1 104 367 FALSE

Rv2497c (Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)) is predicted to be co-regulated in modules bicluster_0158 with residual 0.52 and bicluster_0335 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 20,000.00 and 33,000.00 for bicluster_0158 and 57.00 and 1,600.00 for bicluster_0335 respectively.

These modules are enriched for following go terms: pyruvate dehydrogenase activity, pyruvate dehydrogenase (acetyl-transferr..., oxidoreductase activity, acting on the a... .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:57
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17889 MT2572 480
Product (LegacyBRC) Product (RefSeq)
PROBABLE PYRUVATE DEHYDROGENASE E1 COMPONENT [ALPHA SUBUNIT] PDHA [PYRUVATE DECARBOXYLASE] [PYRUVATE DEHYDROGENASE] [PYRUVIC DEHYDROGENASE] pyruvate dehydrogenase E1 component alpha subunit PdhA
Operon # Operon
1644 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Citrate cycle (TCA cycle)

35
Total items in this category:  

KEGG

Valine, leucine and isoleucine biosynthesis

21
Total items in this category:  

KEGG

Pyruvate metabolism

40
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609634 NP_217013.1 Run
GO:0003863

3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity

3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity

Details: 
Catalysis of the reaction: 3-methyl-2-oxobutanoate + lipoamide = S-(2-methylpropanoyl)dihydrolipoamide + CO2.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0004739

pyruvate dehydrogenase (acetyl-transferring) activity

pyruvate dehydrogenase (acetyl-transferring) activity

Details: 
Catalysis of the reaction: pyruvate + lipoamide = S-acetyldihydrolipoamide + CO2.
GO Category: 
molecular_function
6
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.080000 4.42

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: