Rv2524c [Acyl-carrier-protein] acetyl transferase of FASI (EC 2.3.1.38) / Enoyl-[acyl-carrier-protein] reductase of FASI (EC 1.3.1.9) / 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase of FASI (EC 4.2.1.61) / [Acyl-carrier-protein] malonyl transferase of FASI (EC 2.3.1.39) / [Acyl-carrier-protein] palmitoyl transferase of FASI (EC 2.3.1.-) / Acyl carrier protein of FASI / 3-oxoacyl-[acyl-carrier-protein] reductase of FASI (EC 1.1.1.100) / 3-oxoacyl-[acyl-carrier-protein] synthase of FASI (EC 2.3.1.41)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2524c fas [Acyl-carrier-protein] acetyl transferase of FASI (EC 2.3.1.38) / Enoyl-[acyl-carrier-protein]... CDS 2840123 2849332 - 9 210 3 069 FALSE

Rv2524c ([Acyl-carrier-protein] acetyl transferase of FASI (EC 2.3.1.38) / Enoyl-[acyl-carrier-protein] reductase of FASI (EC 1.3.1.9) / 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase of FASI (EC 4.2.1.61) / [Acyl-carrier-protein] malonyl transferase of FASI (EC 2.3.1.39) / [Acyl-carrier-protein] palmitoyl transferase of FASI (EC 2.3.1.-) / Acyl carrier protein of FASI / 3-oxoacyl-[acyl-carrier-protein] reductase of FASI (EC 1.1.1.100) / 3-oxoacyl-[acyl-carrier-protein] synthase of FASI (EC 2.3.1.41)) is predicted to be co-regulated in modules bicluster_0123 with residual 0.55 and bicluster_0237 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.70 and 4,200.00 for bicluster_0123 and 9.90 and 3,200.00 for bicluster_0237 respectively.

These modules are enriched for following go terms: small molecule biosynthetic process, single-organism biosynthetic process, fatty acid biosynthetic process, carboxylic acid metabolic process, fatty acid synthase activity fatty acid biosynthetic process, monocarboxylic acid biosynthetic process, fatty acid metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 4 of 4
ChipSeq TF Differential Expression Distance Expression pvalue Type
TetR/ACRR family transcriptional regulator
No 38 -0.37 0.854651 Primary.TSS
Transcriptional regulator, AraC family
No -136 -0.13 0.954426 Primary.TSS
Transcriptional regulator, TetR family
No 51 0.21 0.59644 Primary.TSS
Transcriptional regulator, TetR family
No -138 0.27 0.693421 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0123
e.value: 
2.7
Motif Bicluster: 
e.value: 
4200
Motif Bicluster: 
0.55
bicluster_0237
e.value: 
9.9
Motif Bicluster: 
e.value: 
3200
Motif Bicluster: 
0.49
Product (LegacyBRC) Product (RefSeq)
PROBABLE FATTY ACID SYNTHASE FAS [FATTY ACID SYNTHETASE] fatty acid synthase
Operon # Operon
1662
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Transferring groups other than amino-acyl groups. Anthocyanin biosynthesis, Glycerophospholipid metabolism, Fatty acid biosynthesis, Glycosphingolipid biosynthesis - ganglio series, Tropane, piperidine and pyridine alkaloid biosynthesis, Isoquinoline alkaloid biosynthesis, Betalain biosynthesis, Lipopolysaccharide biosynthesis, Benzoate degradation via hydroxylation, Fatty acid biosynthesis, Fatty acid biosynthesis, Carotenoid biosynthesis, Methane metabolism, Naphthalene and anthracene degradation, Histidine metabolism, Ethylbenzene degradation, Diterpenoid biosynthesis, Biosynthesis of type II polyketide backbone, Fatty acid biosynthesis, 2,4-Dichlorobenzoate degradation, Ether lipid metabolism, Stilbenoid, diarylheptanoid and gingerol biosynthesis, Lysine degradation, Butanoate metabolism, 1,4-Dichlorobenzene degradation, Arginine and proline metabolism, Fatty acid biosynthesis, Tyrosine metabolism, Limonene and pinene degradation, Fatty acid biosynthesis, Tetracycline biosynthesis

PATRIC

[Acyl-carrier-protein] S-malonyltransferase Anthocyanin biosynthesis, Glycerophospholipid metabolism, Fatty acid biosynthesis, Glycosphingolipid biosynthesis - ganglio series, Tropane, piperidine and pyridine alkaloid biosynthesis, Isoquinoline alkaloid biosynthesis, Betalain biosynthesis, Lipopolysaccharide biosynthesis, Benzoate degradation via hydroxylation, Fatty acid biosynthesis, Fatty acid biosynthesis, Carotenoid biosynthesis, Methane metabolism, Naphthalene and anthracene degradation, Histidine metabolism, Ethylbenzene degradation, Diterpenoid biosynthesis, Biosynthesis of type II polyketide backbone, Fatty acid biosynthesis, 2,4-Dichlorobenzoate degradation, Ether lipid metabolism, Stilbenoid, diarylheptanoid and gingerol biosynthesis, Lysine degradation, Butanoate metabolism, 1,4-Dichlorobenzene degradation, Arginine and proline metabolism, Fatty acid biosynthesis, Tyrosine metabolism, Limonene and pinene degradation, Fatty acid biosynthesis, Tetracycline biosynthesis

PATRIC

3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase Anthocyanin biosynthesis, Glycerophospholipid metabolism, Fatty acid biosynthesis, Glycosphingolipid biosynthesis - ganglio series, Tropane, piperidine and pyridine alkaloid biosynthesis, Isoquinoline alkaloid biosynthesis, Betalain biosynthesis, Lipopolysaccharide biosynthesis, Benzoate degradation via hydroxylation, Fatty acid biosynthesis, Fatty acid biosynthesis, Carotenoid biosynthesis, Methane metabolism, Naphthalene and anthracene degradation, Histidine metabolism, Ethylbenzene degradation, Diterpenoid biosynthesis, Biosynthesis of type II polyketide backbone, Fatty acid biosynthesis, 2,4-Dichlorobenzoate degradation, Ether lipid metabolism, Stilbenoid, diarylheptanoid and gingerol biosynthesis, Lysine degradation, Butanoate metabolism, 1,4-Dichlorobenzene degradation, Arginine and proline metabolism, Fatty acid biosynthesis, Tyrosine metabolism, Limonene and pinene degradation, Fatty acid biosynthesis, Tetracycline biosynthesis

PATRIC

3-oxoacyl-[acyl-carrier-protein] reductase Anthocyanin biosynthesis, Glycerophospholipid metabolism, Fatty acid biosynthesis, Glycosphingolipid biosynthesis - ganglio series, Tropane, piperidine and pyridine alkaloid biosynthesis, Isoquinoline alkaloid biosynthesis, Betalain biosynthesis, Lipopolysaccharide biosynthesis, Benzoate degradation via hydroxylation, Fatty acid biosynthesis, Fatty acid biosynthesis, Carotenoid biosynthesis, Methane metabolism, Naphthalene and anthracene degradation, Histidine metabolism, Ethylbenzene degradation, Diterpenoid biosynthesis, Biosynthesis of type II polyketide backbone, Fatty acid biosynthesis, 2,4-Dichlorobenzoate degradation, Ether lipid metabolism, Stilbenoid, diarylheptanoid and gingerol biosynthesis, Lysine degradation, Butanoate metabolism, 1,4-Dichlorobenzene degradation, Arginine and proline metabolism, Fatty acid biosynthesis, Tyrosine metabolism, Limonene and pinene degradation, Fatty acid biosynthesis, Tetracycline biosynthesis

PATRIC

Enoyl-[acyl-carrier-protein] reductase (NADH) Anthocyanin biosynthesis, Glycerophospholipid metabolism, Fatty acid biosynthesis, Glycosphingolipid biosynthesis - ganglio series, Tropane, piperidine and pyridine alkaloid biosynthesis, Isoquinoline alkaloid biosynthesis, Betalain biosynthesis, Lipopolysaccharide biosynthesis, Benzoate degradation via hydroxylation, Fatty acid biosynthesis, Fatty acid biosynthesis, Carotenoid biosynthesis, Methane metabolism, Naphthalene and anthracene degradation, Histidine metabolism, Ethylbenzene degradation, Diterpenoid biosynthesis, Biosynthesis of type II polyketide backbone, Fatty acid biosynthesis, 2,4-Dichlorobenzoate degradation, Ether lipid metabolism, Stilbenoid, diarylheptanoid and gingerol biosynthesis, Lysine degradation, Butanoate metabolism, 1,4-Dichlorobenzene degradation, Arginine and proline metabolism, Fatty acid biosynthesis, Tyrosine metabolism, Limonene and pinene degradation, Fatty acid biosynthesis, Tetracycline biosynthesis

PATRIC

Beta-ketoacyl-acyl-carrier-protein synthase I Anthocyanin biosynthesis, Glycerophospholipid metabolism, Fatty acid biosynthesis, Glycosphingolipid biosynthesis - ganglio series, Tropane, piperidine and pyridine alkaloid biosynthesis, Isoquinoline alkaloid biosynthesis, Betalain biosynthesis, Lipopolysaccharide biosynthesis, Benzoate degradation via hydroxylation, Fatty acid biosynthesis, Fatty acid biosynthesis, Carotenoid biosynthesis, Methane metabolism, Naphthalene and anthracene degradation, Histidine metabolism, Ethylbenzene degradation, Diterpenoid biosynthesis, Biosynthesis of type II polyketide backbone, Fatty acid biosynthesis, 2,4-Dichlorobenzoate degradation, Ether lipid metabolism, Stilbenoid, diarylheptanoid and gingerol biosynthesis, Lysine degradation, Butanoate metabolism, 1,4-Dichlorobenzene degradation, Arginine and proline metabolism, Fatty acid biosynthesis, Tyrosine metabolism, Limonene and pinene degradation, Fatty acid biosynthesis, Tetracycline biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid biosynthesis

13
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609661 NP_217040.1 Run
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: