Rv2540c Chorismate synthase (EC 4.2.3.5)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2540c aroF Chorismate synthase (EC 4.2.3.5) CDS 2863207 2864412 - 1 206 401 FALSE

Rv2540c (Chorismate synthase (EC 4.2.3.5)) is predicted to be co-regulated in modules bicluster_0399 with residual 0.58 and bicluster_0460 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 570.00 and 670.00 for bicluster_0399 and 55.00 and 160.00 for bicluster_0460 respectively.

These modules are enriched for following go terms: branched-chain amino acid metabolic proc... branched-chain amino acid metabolic proc..., translation, acetolactate synthase activity, anion transmembrane-transporting ATPase ..., structural constituent of ribosome.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Chorismate synthase chorismate synthase
Operon # Operon
1670 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Chorismate synthase Phenylalanine, tyrosine and tryptophan biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Phenylalanine, tyrosine and tryptophan biosynthesis

18
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609677 NP_217056.1 Run
GO:0004107

chorismate synthase activity

chorismate synthase activity

Details: 
Catalysis of the reaction: 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0004107

chorismate synthase activity

chorismate synthase activity

Details: 
Catalysis of the reaction: 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0010181

FMN binding

FMN binding

Details: 
Interacting selectively and non-covalently with FMN, flavin mononucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0016651

oxidoreductase activity, acting on NADH or NADPH

oxidoreductase activity, acting on NADH or NADPH

Details: 
Catalysis of an oxidation-reduction (redox) reaction in which NADH or NADPH acts as a hydrogen or electron donor and reduces a hydrogen or electron acceptor.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0051287

NAD binding

NAD binding

Details: 
Interacting selectively and non-covalently with nicotinamide adenine dinucleotide, a coenzyme involved in many redox and biosynthetic reactions; binding may be to either the oxidized form, NAD+, or the reduced form, NADH.
GO Category: 
molecular_function
3
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: