Rv2579 Haloalkane dehalogenase (EC 3.8.1.5)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2579 dhaA Haloalkane dehalogenase (EC 3.8.1.5) CDS 2903639 2904541 + 903 300 FALSE

Rv2579 (Haloalkane dehalogenase (EC 3.8.1.5)) is predicted to be co-regulated in modules bicluster_0027 with residual 0.56 and bicluster_0564 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.04 for bicluster_0027 and 0.55 and 7.40 for bicluster_0564 respectively.

These modules are enriched for following go terms: disaccharide metabolic process, trehalose metabolic process, trehalose biosynthetic process, disaccharide biosynthetic process, carbohydrate biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
DNA-binding response regulator
Repressed -21 -0.61 0.0259372 CDS
Transcriptional regulator, TetR family
Repressed -54 -0.87 0.00968437 CDS
Motif 1 Motif 2 Residual
bicluster_0027
e.value: 
0.0000000000000019
Motif Bicluster: 
e.value: 
0.036
Motif Bicluster: 
0.56
bicluster_0564
e.value: 
0.55
Motif Bicluster: 
e.value: 
7.4
Motif Bicluster: 
0.54
Product (LegacyBRC) Product (RefSeq)
Haloalkane dehalogenase 3 haloalkane dehalogenase
Operon # Operon
1694
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Chlorocyclohexane and chlorobenzene degradation

7
Total items in this category:  

KEGG

Chloroalkane and chloroalkene degradation

13
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117001 YP_177890.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0018786

haloalkane dehalogenase activity

haloalkane dehalogenase activity

Details: 
Catalysis of the reaction: 1-haloalkane + H2O = a primary alcohol + halide.
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.020000 0.90

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: