Rv2581c Hydroxyacylglutathione hydrolase (EC 3.1.2.6)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2581c Hydroxyacylglutathione hydrolase (EC 3.1.2.6) CDS 2906089 2906763 - 675 224 FALSE

Rv2581c (Hydroxyacylglutathione hydrolase (EC 3.1.2.6)) is predicted to be co-regulated in modules bicluster_0305 with residual 0.55 and bicluster_0399 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.07 for bicluster_0305 and 570.00 and 670.00 for bicluster_0399 respectively.

These modules are enriched for following go terms: branched-chain amino acid metabolic proc....

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
No 19 -0.22 0.999609 Primary.TSS
DNA-binding response regulator
No -35 -0.52 0.00000361 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0305
e.value: 
0.000000000067
Motif Bicluster: 
e.value: 
0.069
Motif Bicluster: 
0.55
bicluster_0399
e.value: 
570
Motif Bicluster: 
e.value: 
670
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv2581c_MT2658 glyoxalase II
Operon # Operon
1695 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Hydroxyacylglutathione hydrolase Pyruvate metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609718 NP_217097.1 Run
GO:0004416

hydroxyacylglutathione hydrolase activity

hydroxyacylglutathione hydrolase activity

Details: 
Catalysis of the reaction: (S)-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.
GO Category: 
molecular_function
3
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.500000 0.27

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: