Rv2589 Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2589 gabT Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19) CDS 2916360 2917709 + 1 350 449 FALSE

Rv2589 (Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)) is predicted to be co-regulated in modules bicluster_0097 with residual 0.50 and bicluster_0482 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.37 for bicluster_0097 and 0.02 and 0.93 for bicluster_0482 respectively.

These modules are enriched for following go terms: monovalent inorganic cation transmembran..., inorganic cation transmembrane transport... cellular macromolecule biosynthetic proc..., macromolecule biosynthetic process, macromolecule metabolic process, cytoplasm.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:57
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18723 MT2666 2902
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
transcriptional regulator, ArsR family
No -143 0.42 0.0000000113 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0097
e.value: 
0.0000086
Motif Bicluster: 
e.value: 
0.37
Motif Bicluster: 
0.50
bicluster_0482
e.value: 
0.019
Motif Bicluster: 
e.value: 
0.93
Motif Bicluster: 
0.61
Product (LegacyBRC) Product (RefSeq)
4-aminobutyrate aminotransferase 4-aminobutyrate aminotransferase
Operon # Operon
1700
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Alanine, aspartate and glutamate metabolism

26
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

beta-Alanine metabolism

37
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609726 NP_217105.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.160000 1.10

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: