Rv2702 Polyphosphate glucokinase (EC 2.7.1.63)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2702 ppgK Polyphosphate glucokinase (EC 2.7.1.63) CDS 3016858 3017655 + 798 265 FALSE

Rv2702 (Polyphosphate glucokinase (EC 2.7.1.63)) is predicted to be co-regulated in modules bicluster_0024 with residual 0.54 and bicluster_0510 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1.30 and 1.50 for bicluster_0024 and 0.00 and 0.03 for bicluster_0510 respectively.

These modules are enriched for following go terms: iron ion transport, transition metal ion transport.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:17
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14899 MT2776 197
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0024
e.value: 
1.3
Motif Bicluster: 
e.value: 
1.5
Motif Bicluster: 
0.54
bicluster_0510
e.value: 
0.000000033
Motif Bicluster: 
e.value: 
0.032
Motif Bicluster: 
0.59
Product (LegacyBRC) Product (RefSeq)
Polyphosphate glucokinase polyphosphate glucokinase PPGK (polyphosphate-glucose phosphotransferase)
Operon # Operon
1773
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609839 NP_217218.1 Run
GO:0047330

polyphosphate-glucose phosphotransferase activity

polyphosphate-glucose phosphotransferase activity

Details: 
Catalysis of the reaction: beta-D-glucose + long chain polyphosphate = glucose-6-phosphate + long chain polyphosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0004340

glucokinase activity

glucokinase activity

Details: 
Catalysis of the reaction: ATP + D-glucose = ADP + D-glucose-6-phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0047330

polyphosphate-glucose phosphotransferase activity

polyphosphate-glucose phosphotransferase activity

Details: 
Catalysis of the reaction: beta-D-glucose + long chain polyphosphate = glucose-6-phosphate + long chain polyphosphate.
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.010000 0.60

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: