Rv2702 Polyphosphate glucokinase (EC 2.7.1.63)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2702 ppgK Polyphosphate glucokinase (EC 2.7.1.63) CDS 3016858 3017655 + 798 265 FALSE

Rv2702 (Polyphosphate glucokinase (EC 2.7.1.63)) is predicted to be co-regulated in modules bicluster_0024 with residual 0.54 and bicluster_0510 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1.30 and 1.50 for bicluster_0024 and 0.00 and 0.03 for bicluster_0510 respectively.

These modules are enriched for following go terms: iron ion transport, transition metal ion transport.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:17
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14899 MT2776 197
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0024
e.value: 
1.3
Motif Bicluster: 
e.value: 
1.5
Motif Bicluster: 
0.54
bicluster_0510
e.value: 
0.000000033
Motif Bicluster: 
e.value: 
0.032
Motif Bicluster: 
0.59
Product (LegacyBRC) Product (RefSeq)
Polyphosphate glucokinase polyphosphate glucokinase PPGK (polyphosphate-glucose phosphotransferase)
Operon # Operon
1773 Rv2702
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609839 NP_217218.1 Run
GO:0047330

polyphosphate-glucose phosphotransferase activity

polyphosphate-glucose phosphotransferase activity

Details: 
Catalysis of the reaction: beta-D-glucose + long chain polyphosphate = glucose-6-phosphate + long chain polyphosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0004340

glucokinase activity

glucokinase activity

Details: 
Catalysis of the reaction: ATP + D-glucose = ADP + D-glucose-6-phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0047330

polyphosphate-glucose phosphotransferase activity

polyphosphate-glucose phosphotransferase activity

Details: 
Catalysis of the reaction: beta-D-glucose + long chain polyphosphate = glucose-6-phosphate + long chain polyphosphate.
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.010000 0.60

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.