Rv2725c GTP-binding protein HflX

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2725c hflX GTP-binding protein HflX CDS 3037427 3038914 - 1 488 495 FALSE

Rv2725c (GTP-binding protein HflX) is predicted to be co-regulated in modules bicluster_0232 with residual 0.52 and bicluster_0301 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2,200.00 and 5,400.00 for bicluster_0232 and 0.00 and 4.40 for bicluster_0301 respectively.

These modules are enriched for following go terms: primary metabolic process, cellular metabolic process, cell, cell part divalent metal ion transport, divalent inorganic cation transport, transition metal ion transport, divalent inorganic cation transmembrane ....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.998 3038857 3038914
Last update: 10/16/2017 - 14:17
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18883 MT2797 3241
Product (LegacyBRC) Product (RefSeq)
PROBABLE GTP-BINDING PROTEIN HFLX GTP-binding protein HflX
Operon # Operon
1793 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609862 NP_217241.1 Run
GO:0005525

GTP binding

GTP binding

Details: 
Interacting selectively and non-covalently with GTP, guanosine triphosphate.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0005622

intracellular

intracellular

Details: 
The living contents of a cell; the matter contained within (but not including) the plasma membrane, usually taken to exclude large vacuoles and masses of secretory or ingested material. In eukaryotes it includes the nucleus and cytoplasm.
GO Category: 
cellular_component
65
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.450000 1.00

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: