Rv2754c Thymidylate synthase thyX (EC 2.1.1.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2754c thyX Thymidylate synthase thyX (EC 2.1.1.-) CDS 3067193 3067945 - 753 250 FALSE

Rv2754c (Thymidylate synthase thyX (EC 2.1.1.-)) is predicted to be co-regulated in modules bicluster_0052 with residual 0.48 and bicluster_0317 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 460.00 and 2,100.00 for bicluster_0052 and 12,000.00 and 12,000.00 for bicluster_0317 respectively.

These modules are enriched for following go terms: flavin adenine dinucleotide binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Thymidylate synthase thyX FAD-dependent thymidylate synthase
Operon # Operon
1812
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

One carbon pool by folate

13
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609891 NP_217270.1 Run
GO:0004799

thymidylate synthase activity

thymidylate synthase activity

Details: 
Catalysis of the reaction: 5,10-methylenetetrahydrofolate + dUMP = 7,8-dihydrofolate + thymidylate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0050660

flavin adenine dinucleotide binding

flavin adenine dinucleotide binding

Details: 
Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0050797

thymidylate synthase (FAD) activity

thymidylate synthase (FAD) activity

Details: 
Catalysis of the reaction: 5,10-methylenetetrahydrofolate + dUMP + FADH(2) = (6S)-5,6,7,8-tetrahydrofolate + FAD + H(+) + thymidylate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0070402

NADPH binding

NADPH binding

Details: 
Interacting selectively and non-covalently with the reduced form, NADPH, of nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions.
GO Category: 
molecular_function
6
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: