Rv2764c Thymidylate synthase (EC 2.1.1.45)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2764c thyA Thymidylate synthase (EC 2.1.1.45) CDS 3073680 3074471 - 792 263 FALSE

Rv2764c (Thymidylate synthase (EC 2.1.1.45)) is predicted to be co-regulated in modules bicluster_0031 with residual 0.69 and bicluster_0512 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0031 and 1,300.00 and 6,600.00 for bicluster_0512 respectively.

These modules are enriched for following go terms: damaged DNA binding, cysteine-type endopeptidase activity, cysteine-type peptidase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-2.274 116 3074355 -125 3074480 3074480 3074471
Product (LegacyBRC) Product (RefSeq)
Thymidylate synthase thymidylate synthase
Operon # Operon
1816
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Thymidylate synthase Pyrimidine metabolism, One carbon pool by folate
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

One carbon pool by folate

13
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609901 NP_217280.1 Run
GO:0004799

thymidylate synthase activity

thymidylate synthase activity

Details: 
Catalysis of the reaction: 5,10-methylenetetrahydrofolate + dUMP = 7,8-dihydrofolate + thymidylate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0004799

thymidylate synthase activity

thymidylate synthase activity

Details: 
Catalysis of the reaction: 5,10-methylenetetrahydrofolate + dUMP = 7,8-dihydrofolate + thymidylate.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: