Rv2770c PPE family protein

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2770c PPE44 PPE family protein CDS 3079309 3080457 - 1 149 382 FALSE

Rv2770c (PPE family protein) is predicted to be co-regulated in modules bicluster_0438 with residual 0.51 and bicluster_0565 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.50 and 3,200.00 for bicluster_0438 and 0.16 and 35.00 for bicluster_0565 respectively.

These modules are enriched for following go terms: porphyrin-containing compound metabolic ..., porphyrin-containing compound biosynthet..., cofactor biosynthetic process, sulfur amino acid biosynthetic process, sulfur amino acid metabolic process, cofactor metabolic process .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.525 94 3080509 3080415 3080457
Last update: 10/16/2017 - 14:17
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17990 MT2840 1072
Product (LegacyBRC) Product (RefSeq)
PPE FAMILY PROTEIN PPE family protein
Operon # Operon
1821
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117024 YP_177677.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.290000 0.90

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: