Rv2773c Dihydrodipicolinate reductase (EC 1.3.1.26)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2773c dapB Dihydrodipicolinate reductase (EC 1.3.1.26) CDS 3081604 3082341 - 738 245 FALSE

Rv2773c (Dihydrodipicolinate reductase (EC 1.3.1.26)) is predicted to be co-regulated in modules bicluster_0325 with residual 0.54 and bicluster_0365 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 72.00 for bicluster_0325 and 0.00 and 8,900.00 for bicluster_0365 respectively.

These modules are enriched for following go terms: gene expression, macromolecule metabolic process, cellular macromolecule biosynthetic proc..., macromolecule biosynthetic process, ribosome, ribonucleoprotein complex, non-membrane-bounded organelle, intracellular non-membrane-bounded organ..., nucleotidyltransferase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0325
e.value: 
0.0049
Motif Bicluster: 
e.value: 
72
Motif Bicluster: 
0.54
bicluster_0365
e.value: 
0.0049
Motif Bicluster: 
e.value: 
8900
Motif Bicluster: 
0.53
Product (LegacyBRC) Product (RefSeq)
Dihydrodipicolinate reductase dihydrodipicolinate reductase
Operon # Operon
1823 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Dihydrodipicolinate reductase Lysine biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Lysine biosynthesis

15
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609910 NP_217289.1 Run
GO:0008839

dihydrodipicolinate reductase activity

dihydrodipicolinate reductase activity

Details: 
Catalysis of the reaction: 2,3,4,5-tetrahydrodipicolinate + NADP+ = 2,3-dihydrodipicolinate + NADPH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008839

dihydrodipicolinate reductase activity

dihydrodipicolinate reductase activity

Details: 
Catalysis of the reaction: 2,3,4,5-tetrahydrodipicolinate + NADP+ = 2,3-dihydrodipicolinate + NADPH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0019877

diaminopimelate biosynthetic process

diaminopimelate biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of diaminopimelate, both as an intermediate in lysine biosynthesis and as a component (as meso-diaminopimelate) of the peptidoglycan of Gram-negative bacterial cell walls.
GO Category: 
biological_process
1
Total items in this category:  
GO:0051260

protein homooligomerization

protein homooligomerization

Details: 
The process of creating protein oligomers, compounds composed of a small number, usually between three and ten, of identical component monomers. Oligomers may be formed by the polymerization of a number of monomers or the depolymerization of a large protein polymer.
GO Category: 
biological_process
31
Total items in this category:  
GO:0070404

NADH binding

NADH binding

Details: 
Interacting selectively and non-covalently with the reduced form, NADH, of nicotinamide adenine dinucleotide, a coenzyme involved in many redox and biosynthetic reactions.
GO Category: 
molecular_function
3
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: