Rv2783c Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2783c gpsI Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) CDS 3090339 3092597 - 2 259 752 FALSE

Rv2783c (Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)) is predicted to be co-regulated in modules bicluster_0271 with residual 0.56 and bicluster_0528 with residual 0.42.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 0.03 for bicluster_0271 and 0.00 and 47.00 for bicluster_0528 respectively.

These modules are enriched for following go terms: carboxylic acid biosynthetic process, organic acid biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Organic hydroperoxide resistance transcriptional regulator
No -37 0.12 0.583282 Primary.TSS
ESX-1 secreted protein regulator EspR
Repressed 20 -0.73 0.000000000202 Primary.TSS
Transcriptional regulator, TetR family
No -45 0.08 0.814608 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0271
e.value: 
0.013
Motif Bicluster: 
e.value: 
0.027
Motif Bicluster: 
0.56
bicluster_0528
e.value: 
0.00023
Motif Bicluster: 
e.value: 
47
Motif Bicluster: 
0.42
Product (LegacyBRC) Product (RefSeq)
Polyribonucleotide nucleotidyltransferase polynucleotide phosphorylase/polyadenylase
Operon # Operon
1830 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Polyribonucleotide nucleotidyltransferase Pyrimidine metabolism, Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

RNA degradation

10
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609920 NP_217299.1 Run
GO:0004654

polyribonucleotide nucleotidyltransferase activity

polyribonucleotide nucleotidyltransferase activity

Details: 
Catalysis of the reaction: ATP + RNA(n) = diphosphate + RNA(n+1).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: