Rv2839c Translation initiation factor 2

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2839c infB Translation initiation factor 2 CDS 3145171 3147873 - 2 703 900 FALSE

Rv2839c (Translation initiation factor 2) is predicted to be co-regulated in modules bicluster_0428 with residual 0.47 and bicluster_0528 with residual 0.42.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.52 for bicluster_0428 and 0.00 and 47.00 for bicluster_0528 respectively.

These modules are enriched for following go terms: ATPase activity, coupled, ATP-dependent DNA helicase activity, ATPase activity, nuclease activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
DNA-binding response regulator TrcR
No -11 0.09 0.82975 Primary.TSS
Transcriptional regulator kstR (Rv3574), TetR family
No 4 -0.16 0.227413 Primary.TSS
Two component DNA binding Transcriptional regulatory protein TcrA
No -15 -0.02 0.96398 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0428
e.value: 
0.00000095
Motif Bicluster: 
e.value: 
0.52
Motif Bicluster: 
0.47
bicluster_0528
e.value: 
0.00023
Motif Bicluster: 
e.value: 
47
Motif Bicluster: 
0.42
Product (LegacyBRC) Product (RefSeq)
Translation initiation factor IF-2 translation initiation factor IF-2
Operon # Operon
1859 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609976 NP_217355.1 Run
GO:0006413

translational initiation

translational initiation

Details: 
The process preceding formation of the peptide bond between the first two amino acids of a protein. This includes the formation of a complex of the ribosome, mRNA, and an initiation complex that contains the first aminoacyl-tRNA.
GO Category: 
biological_process
3
Total items in this category:  
GO:0006412

translation

translation

Details: 
The cellular metabolic process in which a protein is formed, using the sequence of a mature mRNA molecule to specify the sequence of amino acids in a polypeptide chain. Translation is mediated by the ribosome, and begins with the formation of a ternary complex between aminoacylated initiator methionine tRNA, GTP, and initiation factor 2, which subsequently associates with the small subunit of the ribosome and an mRNA. Translation ends with the release of a polypeptide chain from the ribosome.
GO Category: 
biological_process
62
Total items in this category:  
GO:0003743

translation initiation factor activity

translation initiation factor activity

Details: 
Functions in the initiation of ribosome-mediated translation of mRNA into a polypeptide.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005525

GTP binding

GTP binding

Details: 
Interacting selectively and non-covalently with GTP, guanosine triphosphate.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: