Rv2848c Cobyrinic acid A,C-diamide synthase

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2848c cobB Cobyrinic acid A,C-diamide synthase CDS 3156148 3157521 - 1 374 457 FALSE

Rv2848c (Cobyrinic acid A,C-diamide synthase) is predicted to be co-regulated in modules bicluster_0405 with residual 0.59 and bicluster_0497 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.58 and 2.90 for bicluster_0405 and 310.00 and 800.00 for bicluster_0497 respectively.

These modules are enriched for following go terms: alcohol metabolic process, phospholipid metabolic process, phospholipid biosynthetic process 3-hydroxyacyl-CoA dehydrogenase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.905 3157527 3157521
Product (LegacyBRC) Product (RefSeq)
Cobyrinic acid AC-diamide synthase cobyrinic acid a,c-diamide synthase
Operon # Operon
1865 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Porphyrin and chlorophyll metabolism

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Metabolic pathways

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BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15609985 NP_217364.1 Run

cobalamin biosynthetic process

cobalamin biosynthetic process

The chemical reactions and pathways resulting in the formation of cobalamin (vitamin B12), a water-soluble vitamin characterized by possession of a corrin nucleus containing a cobalt atom.
GO Category: 
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catalytic activity

catalytic activity

Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO Category: 
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plasma membrane

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
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No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.780000 1.12

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: