Rv2855 NADPH-dependent mycothiol reductase Mtr

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2855 mtr NADPH-dependent mycothiol reductase Mtr CDS 3165205 3166584 + 1 380 459 FALSE

Rv2855 (NADPH-dependent mycothiol reductase Mtr) is predicted to be co-regulated in modules bicluster_0034 with residual 0.54 and bicluster_0056 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 57.00 and 260.00 for bicluster_0034 and 0.11 and 16,000.00 for bicluster_0056 respectively.

These modules are enriched for following go terms: phosphotransferase activity, for other s... oxidoreductase activity, acting on a sul..., helicase activity, oxidoreductase activity, acting on a sul....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
NADPH-DEPENDENT MYCOTHIOL REDUCTASE MTR mycothione reductase
Operon # Operon
1867 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glutathione metabolism

14
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117030 YP_177910.1 Run
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0050627

mycothione reductase activity

mycothione reductase activity

Details: 
Catalysis of the reaction: NAD(P)+ + mycothiol = NAD(P)H + H+ + mycothione.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0050660

flavin adenine dinucleotide binding

flavin adenine dinucleotide binding

Details: 
Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0055114

oxidation-reduction process

oxidation-reduction process

Details: 
A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO Category: 
biological_process
19
Total items in this category:  
GO:0070402

NADPH binding

NADPH binding

Details: 
Interacting selectively and non-covalently with the reduced form, NADPH, of nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions.
GO Category: 
molecular_function
6
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.530000 1.25

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: