Rv2856 HoxN/HupN/NixA family nickel/cobalt transporter

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2856 nicT HoxN/HupN/NixA family nickel/cobalt transporter CDS 3166684 3167802 + 1 119 372 FALSE

Rv2856 (HoxN/HupN/NixA family nickel/cobalt transporter) is predicted to be co-regulated in modules bicluster_0450 with residual 0.52 and bicluster_0565 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.02 and 190.00 for bicluster_0450 and 0.16 and 35.00 for bicluster_0565 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
POSSIBLE NICKEL-TRANSPORT INTEGRAL MEMBRANE PROTEIN NICT nickel-transport integral membrane protein
Operon # Operon
1868
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609993 NP_217372.1 Run
GO:0030001

metal ion transport

metal ion transport

Details: 
The directed movement of metal ions, any metal ion with an electric charge, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
GO Category: 
biological_process
3
Total items in this category:  
GO:0016021

integral to membrane

integral to membrane

Details: 
Penetrating at least one phospholipid bilayer of a membrane. May also refer to the state of being buried in the bilayer with no exposure outside the bilayer. When used to describe a protein, indicates that all or part of the peptide sequence is embedded in the membrane.
GO Category: 
cellular_component
6
Total items in this category:  
GO:0046872

metal ion binding

metal ion binding

Details: 
Interacting selectively and non-covalently with any metal ion.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.640000 1.69

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: