Rv2878c Soluble secreted antigen MPT53 precursor

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2878c mpt53 Soluble secreted antigen MPT53 precursor CDS 3188876 3189397 - 522 173 FALSE

Rv2878c (Soluble secreted antigen MPT53 precursor) is predicted to be co-regulated in modules bicluster_0447 with residual 0.49 and bicluster_0542 with residual 0.60.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2,200.00 and 540.00 for bicluster_0447 and 0.02 and 150.00 for bicluster_0542 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18573 MT2946 2581
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
transcriptional regulator, ArsR family
No 6 0.32 0.139474 CDS
Motif 1 Motif 2 Residual
bicluster_0447
e.value: 
2200
Motif Bicluster: 
e.value: 
540
Motif Bicluster: 
0.49
bicluster_0542
e.value: 
0.019
Motif Bicluster: 
e.value: 
150
Motif Bicluster: 
0.60
Product (LegacyBRC) Product (RefSeq)
Soluble secreted antigen MPT53 soluble secreted antigen MPT53 precursor
Operon # Operon
1882 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610015 NP_217394.1 Run
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0016491

oxidoreductase activity

oxidoreductase activity

Details: 
Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.
GO Category: 
molecular_function
12
Total items in this category:  
GO:0045454

cell redox homeostasis

cell redox homeostasis

Details: 
Any process that maintains the redox environment of a cell or compartment within a cell.
GO Category: 
biological_process
9
Total items in this category:  
GO:0055114

oxidation-reduction process

oxidation-reduction process

Details: 
A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO Category: 
biological_process
19
Total items in this category:  
GO:0003756

protein disulfide isomerase activity

protein disulfide isomerase activity

Details: 
Catalysis of the rearrangement of both intrachain and interchain disulfide bonds in proteins.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0015035

protein disulfide oxidoreductase activity

protein disulfide oxidoreductase activity

Details: 
Catalysis of the reaction: a protein with reduced sulfide groups = a protein with oxidized disulfide bonds.
GO Category: 
molecular_function
7
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.890000 4.95

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: