Rv2879c Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2879c Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-) CDS 3189583 3190152 - 570 189 FALSE

Rv2879c (Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-)) is predicted to be co-regulated in modules bicluster_0047 with residual 0.41 and bicluster_0224 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,300.00 and 3,500.00 for bicluster_0047 and 480.00 and 27.00 for bicluster_0224 respectively.

These modules are enriched for following go terms: regulation of cellular process, regulation of biological process, biological regulation.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.093 218 3189934 -122 305 3190361 3190056 3190152
Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15049 MT2947 1248
Product (LegacyBRC) Product (RefSeq)
Ribosomal RNA large subunit methyltransferase N
Operon # Operon
1883 - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610016 NP_217395.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: