Rv2899c Formate dehydrogenase chain D (EC 1.2.1.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2899c fdhD Formate dehydrogenase chain D (EC 1.2.1.2) CDS 3208576 3209406 - 831 276 FALSE

Rv2899c (Formate dehydrogenase chain D (EC 1.2.1.2)) is predicted to be co-regulated in modules bicluster_0192 with residual 0.54 and bicluster_0415 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 4,500.00 and 12,000.00 for bicluster_0192 and 37.00 and 3,500.00 for bicluster_0415 respectively.

These modules are enriched for following go terms: cellular macromolecular complex assembly, cellular protein complex assembly, protein complex assembly, macromolecular complex assembly, protein complex biogenesis response to stress.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Protein fdhD homolog formate dehydrogenase accessory protein
Operon # Operon
1894 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Formate dehydrogenase Glyoxylate and dicarboxylate metabolism, Methane metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610036 NP_217415.1 Run
GO:0008863

formate dehydrogenase (NAD+) activity

formate dehydrogenase (NAD+) activity

Details: 
Catalysis of the reaction: formate + NAD(+) = CO(2) + NADH.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.620000 1.97

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: