Rv2902c Ribonuclease HII (EC 3.1.26.4)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2902c rnhB Ribonuclease HII (EC 3.1.26.4) CDS 3212162 3212956 - 795 264 FALSE

Rv2902c (Ribonuclease HII (EC 3.1.26.4)) is predicted to be co-regulated in modules bicluster_0285 with residual 0.48 and bicluster_0320 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3,800.00 and 4,600.00 for bicluster_0285 and 12.00 and 350.00 for bicluster_0320 respectively.

These modules are enriched for following go terms: glutamine family amino acid biosynthetic..., glutamine family amino acid metabolic pr....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Ribonuclease HII ribonuclease HII
Operon # Operon
1896 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

DNA replication

14
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610039 NP_217418.1 Run
GO:0004523

ribonuclease H activity

ribonuclease H activity

Details: 
Catalysis of the endonucleolytic cleavage of RNA in RNA-DNA hybrids to 5'-phosphomonoesters.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.820000 1.57

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: