Rv2906c tRNA (Guanine37-N1) -methyltransferase (EC 2.1.1.31)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2906c trmD tRNA (Guanine37-N1) -methyltransferase (EC 2.1.1.31) CDS 3215665 3216357 - 693 230 FALSE

Rv2906c (tRNA (Guanine37-N1) -methyltransferase (EC 2.1.1.31)) is predicted to be co-regulated in modules bicluster_0302 with residual 0.49 and bicluster_0600 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0302 and 2,200.00 and 750.00 for bicluster_0600 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
tRNA [guanine-N[1]-]-methyltransferase tRNA (guanine-N(1)-)-methyltransferase
Operon # Operon
1899 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610043 NP_217422.1 Run
GO:0009019

tRNA (guanine-N1-)-methyltransferase activity

tRNA (guanine-N1-)-methyltransferase activity

Details: 
Catalysis of the reaction: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N1-methylguanine.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: