Rv2941 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2941 fadD28 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) CDS 3283335 3285077 + 1 743 580 FALSE

Rv2941 (Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)) is predicted to be co-regulated in modules bicluster_0243 with residual 0.50 and bicluster_0582 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.06 and 2,000.00 for bicluster_0243 and 0.07 and 0.22 for bicluster_0582 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15123 MT3011 1027
Product (LegacyBRC) Product (RefSeq)
FATTY-ACID-CoA LIGASE FADD28 [FATTY-ACID-CoA SYNTHETASE] [FATTY-ACID-CoA SYNTHASE] acyl-CoA synthetase
Operon # Operon
1918 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Long-chain-fatty-acid--CoA ligase Fatty acid metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610078 NP_217457.1 Run
GO:0004467

long-chain fatty acid-CoA ligase activity

long-chain fatty acid-CoA ligase activity

Details: 
Catalysis of the reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA; a long-chain fatty acid is a fatty acid with a chain length between C13 and C22.
GO Category: 
molecular_function
13
Total items in this category:  
GO:0004321

fatty-acyl-CoA synthase activity

fatty-acyl-CoA synthase activity

Details: 
Catalysis of the reaction: acetyl-CoA + n malonyl-CoA + 2n NADH + 2n NADPH + 4n H+ = a long-chain acyl-CoA + n CoA + n CO2 + 2n NAD+ + 2n NADP+.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008610

lipid biosynthetic process

lipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent.
GO Category: 
biological_process
13
Total items in this category:  
GO:0044119

growth of symbiont in host cell

growth of symbiont in host cell

Details: 
The increase in size or mass of symbiont, occurring in its host's cell. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
50
Total items in this category:  
GO:0052572

response to host immune response

response to host immune response

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detecting the immune response of the host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
62
Total items in this category:  
GO:0070566

adenylyltransferase activity

adenylyltransferase activity

Details: 
Catalysis of the transfer of an adenylyl group to an acceptor.
GO Category: 
molecular_function
6
Total items in this category:  
GO:0071766

Actinobacterium-type cell wall biogenesis

Actinobacterium-type cell wall biogenesis

Details: 
A cellular process that results in the biosynthesis of constituent macromolecules, assembly, and arrangement of constituent parts of a cell wall of the type found in Actinobacteria. The cell wall is the rigid or semi-rigid envelope lying outside the cell membrane. Actinobacterial cell walls contain characteristic mycolic acids, of which some are covalently linked to the cell wall peptidoglycan and others accumulate at the cell surface.
GO Category: 
biological_process
19
Total items in this category:  
GO:0071770

DIM/DIP cell wall layer assembly

DIM/DIP cell wall layer assembly

Details: 
The aggregation, arrangement and bonding together of a set of components, including (phenyl)phthiocerol, phthiodiolone, phthiotriol dimycocerosate and diphthioceranate, to form the DIM/DIP layer of the Actinobacterium-type cell wall.
GO Category: 
biological_process
6
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.070000 1.09

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: