Rv2957 PGL/p-HBAD biosynthesis glycosyltransferase

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2957 PGL/p-HBAD biosynthesis glycosyltransferase CDS 3309470 3310297 + 828 275 FALSE

Rv2957 (PGL/p-HBAD biosynthesis glycosyltransferase) is predicted to be co-regulated in modules bicluster_0162 with residual 0.54 and bicluster_0282 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 45.00 and 5,400.00 for bicluster_0162 and 0.14 and 0.27 for bicluster_0282 respectively.

These modules are enriched for following go terms: cysteine biosynthetic process from serin..., cysteine biosynthetic process, cysteine metabolic process, L-serine metabolic process .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14973 MT3031 1478
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
ESX-1 secreted protein regulator EspR
Repressed 26 -1.44 0.00000208 CDS
No 23 -0.19 0.661693 Antisense.TSS
Motif 1 Motif 2 Residual
bicluster_0162
e.value: 
45
Motif Bicluster: 
e.value: 
5400
Motif Bicluster: 
0.54
bicluster_0282
e.value: 
0.14
Motif Bicluster: 
e.value: 
0.27
Motif Bicluster: 
0.48
Product (LegacyBRC) Product (RefSeq)
PGL_p-HBAD biosynthesis glycosyltransferase Rv2957_MT3031 glycosyl transferase
Operon # Operon
1927
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610094 NP_217473.1 Run
GO:0009247

glycolipid biosynthetic process

glycolipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glycolipid, a class of 1,2-di-O-acylglycerols joined at oxygen 3 by a glycosidic linkage to a carbohydrate part (usually a mono-, di- or tri-saccharide).
GO Category: 
biological_process
17
Total items in this category:  
GO:0016758

transferase activity, transferring hexosyl groups

transferase activity, transferring hexosyl groups

Details: 
Catalysis of the transfer of a hexosyl group from one compound (donor) to another (acceptor).
GO Category: 
molecular_function
7
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.560000 1.02

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: