Rv2964 Formyltetrahydrofolate deformylase (EC 3.5.1.10)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2964 purU Formyltetrahydrofolate deformylase (EC 3.5.1.10) CDS 3316529 3317461 + 933 310 FALSE

Rv2964 (Formyltetrahydrofolate deformylase (EC 3.5.1.10)) is predicted to be co-regulated in modules bicluster_0035 with residual 0.52 and bicluster_0531 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 130.00 for bicluster_0035 and 3,300.00 and 2,400.00 for bicluster_0531 respectively.

These modules are enriched for following go terms: hydroxymethyl-, formyl- and related tran... protein dimerization activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Formyltetrahydrofolate deformylase formyltetrahydrofolate deformylase
Operon # Operon
1934
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glyoxylate and dicarboxylate metabolism

31
Total items in this category:  

KEGG

One carbon pool by folate

13
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610101 NP_217480.1 Run
GO:0008864

formyltetrahydrofolate deformylase activity

formyltetrahydrofolate deformylase activity

Details: 
Catalysis of the reaction: 10-formyltetrahydrofolate + H(2)O = (6S)-5,6,7,8-tetrahydrofolate + formate + H(+).
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.540000 1.61

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: