Rv2981c D-alanine--D-alanine ligase (EC 6.3.2.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2981c ddlA D-alanine--D-alanine ligase (EC 6.3.2.4) CDS 3336796 3337917 - 1 122 373 FALSE

Rv2981c (D-alanine--D-alanine ligase (EC 6.3.2.4)) is predicted to be co-regulated in modules bicluster_0112 with residual 0.53 and bicluster_0520 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.70 and 3.00 for bicluster_0112 and 330.00 and 7,500.00 for bicluster_0520 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
D-alanine--D-alanine ligase D-alanyl-alanine synthetase A
Operon # Operon
1944
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

D-alanine--D-alanine ligase D-Alanine metabolism, Peptidoglycan biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

D-Alanine metabolism

2
Total items in this category:  

KEGG

Peptidoglycan biosynthesis

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610118 NP_217497.1 Run
GO:0008716

D-alanine-D-alanine ligase activity

D-alanine-D-alanine ligase activity

Details: 
Catalysis of the reaction: 2 D-alanine + ATP = D-alanyl-D-alanine + ADP + 2 H(+) + phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0008150

biological_process

biological_process

Details: 
Any process specifically pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms. A process is a collection of molecular events with a defined beginning and end.
GO Category: 
biological_process
66
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: